Drug use to resistance exercise-induced fatigue based on diclofenac diethylamine emulgel efficacy analysis

In this paper, the author study on the effect of drug treatment on sports injury, and makes a comparative analysis of drug effects. In sports, the incidence of various types of injuries is increasing, especially in muscle injury. In the experiment, we compared the effects of three different drugs on the treatment and relief of muscle loss. After 3 weeks, the average optical density of desmin in muscle fiber positive region have decreased, as xiaotong plaster [0.4708 +/- 0.0126], votalin [0.5124 +/- 0.0264] and placebo [0.3856 +/- 0.0312]. It has a certain effect to promote the repair and regeneration of desmin expression by drugs. Through the analysis of the effect of drug intervention on sports injury repair, we can effectively improve the therapeutic effect of sports injury

Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Objetivo. Evaluar la genotoxicidad de N-nitroso dietilamina (NDEA), hidrazida málica (MH) y etil metano sulfonato (EMS), en núcleos de Tradescantia (clona 4430) por medio de la prueba del cometa y de la prueba de mutación rosa, en los pelos estaminales de la misma planta. Material y métodos. Las plantas de Tradescantia (clon 4430) fueron obtenidas del Laboratorio de Citogenética y Mutagénesis del Centro de ciencias de la Atmósfera de la Universidad Nacional Autónoma de México, tratadas con NDEA a 1, 5, 10 mM, MH a 1, 5, 10 mM y EMS a 15, 30 y 45 mM, y utilizadas en la prueba de mutación rosa y en la del cometa, en núcleos celulares de los pelos estaminales. En la primera, la lectura de los pelos estaminales se realizó de acuerdo con el método de Underbrink. En otros estudios, que han aplicado la prueba del cometa en plantas, existe la necesidad de romper la pared celular y separar los núcleos por gradiente de centrifugación; en este caso, los núcleos de las células de los pelos estaminales fueron extraídos por aplastamiento sin aplicar un procedimiento especial para romper la pared, colectados por filtración en una malla de nylon y sometidos a la prueba del cometa. La prueba t de Student se usó para analizar los datos obtenidos. Resultados. Ambas pruebas presentaron una gran sensibilidad a los mutágenos estudiados y hubo una relación evidente dosis-eventos rosa / longitud de la cauda. Aunque la prueba de mutación rosa en Tradescantia fue muy sensible a MH y EMS, no se detectaron dosis bajas de NDEA; en cambio, la prueba del cometa en la misma planta permite detectar fácilmente la actividad de todos los agentes estudiados. Conclusión. La prueba del cometa en los núcleos de las células de los pelos estaminales de Tradescantia es una útil herramienta para los estudios de monitoreo. Además, es simple, sensible y más rápida que la prueba de mutación rosa en la misma planta. El texto completo en inglés de este artículo también está disponible en: http://www.insp.mx/salud/index.htm

mutagenic effect of diethylamine in candida intermedia and C pelliculosa

The effect of diethylamine on C. intermedia survivals was severe at the concentration of 0. 5 ml/100 ml liquid minimal medium. Stimulation in survivals was obtained at the concentration of 0.7 ml DEA/100 ml LMM, while complete lethality was obtained at the concentration of 1 ml DEA. No mutants could be obtained, fore the species, by any of the different DEA concentrations used. The effect of DEA on C. pelliculosa survivals was gradual and mild until the concentration of 0.5 ml DEA/100 ml LMM and severe at the concentration of 0.7 ml DEA. Complete lethality was obtained at the concentration of 1 ml. No mutants were obtained from any of the different DEA concertrations used with C. pelliculosa, i.e., C. pelliculosa is similar to C. mutation by DEA in respect of mutation induction by DEA

mutagenic effect of nitrous acid, acriflavine and diethylamine on streptomyces scabies

The mean survival percentages for the concentrations 0.02 and 0.2 M NaNO2 in liquid MM after incubation for 24 hr at 280 were found to be 4.64 and 3.57, respectively. Out of 161 colonies tested from the concentration of 0.02 M, 64 auxotrophic mutants were isolated. However, out of 150 colonies tested from the concentration of 0.2 M, a total of 74 auxotrphic mutants was isolated. All these mutants reverted to the wild type. The mean survival percentages for the concentrations 10, 20 and 30 mg AC/20 ml solid CM and incubation for 4 days at 280 were 9.33,4.00 and 1.73, respectively. The mean survival percentages for the concentrations 10,20 and 30 mg AC/100 ml liquid MM and incubation for 4 days at 28° were 9.09, 6.66 and 5.64, respectively. The mean survival perecentages for the concentrations 10, 20 and 30 mg AC + 2 mg streptopencid/20 ml solid CM and incubation for 7 days at 28° were 0.67,0.12 and Q respectively. Three biochemical mutants were obtained from a sample of 127 colonies isolated from 30 mg AC + 2 mg streptopencid/20 ml solid CM. The mutants were ad, his and met requiring mutants. The mean survival percentages for the concentrations 0.05 and 0.1 ml DEA/20 ml solid CM and incubation for 4 days at 280 were 1.83 and 0.15, respectively. Twenty biochemical mutants were obtained from conidial suspensions treated with 0.05 ml DEA which gave a survival percentage of 1.83. These mutants were;- 3 his, 4 ad, 2 arg, 2 trypt, 1 ad or lys, 1 met or trypt, 1 ad or trypt, 1 his or trypt, 1 his or arg, 1 his or met and 21eu or trypt. The possibility for obtaining such latter mutants were discussed

Local anaesthetic activity of some new substituted acylamides II.

Six new substituted acylamides, chemically related to lignocaine were studied for local anaesthetic activity and toxicity in mice, frogs and guinea pigs. Only one of these compounds, w-pyrrolidino 2, 3, 5, 6 tetramethyl acetanilide was found to possess potency comparable to lignocaine with a slightly higher therapeutic index. Study of the S.A.R. of this group indicated that by removal of two methyl groups at position 3 and 5 in the above compound, a local anaesthetic with greater potency than lignocaine may be obtained. Further exploration of the potentialities of a compound having pyrrolidine group as a part of basic side chain is indicated.